Long Non-Coding RNA NANCI/NKX2-1 Duplex Impacts Prognosis in Stage I Non-Small-Cell Lung Cancer

BackgroundNANCI, an intergenic long non-coding RNA (lncRNA) is essential for buffering NKX2-1 expression during embryonic development and in adult tissue. We analyzed NANCI and NKX2-1 in human lung embryonic samples and adult lung tissues and evaluated their potential as prognostic markers in stage I non-small cell lung cancer (NSCLC).Methods and resultsNANCI and NKX2-1 expression was assessed by TaqMan assays in 18 human embryonic samples from 8 to 13 weeks, 59 non-tumoral (NT) lung tissue samples, and 98 stage I NSCLC tumor samples. NANCI and NKX2-1 expression in embryonic and NSCLC samples were downregulated in comparison to adult NT tissue. Patients with low expression of NANCI had shorter disease-free survival (DFS) and overall survival (OS) than those with high levels (47.6 vs 69.3 months, P = 0.032 and 57.7 vs 77.6 months, P = 0.021, respectively). When the expression levels of NANCI and NKX2-1 were evaluated in combination, four groups were identified (high NANCI/high NKX2-1, low NANCI/high NKX2-1, high NANCI/low NKX2-1 and low NANCI/low NKX2-1) with differential impact on DFS (P = 0.042) and OS (P = 0.024). Interestingly, the high NANCI/high NKX2-1 duplex group had longer DFS and OS than the other three groups (71.25 vs 46.3 months, P = 0.009 and 81.3 vs 56.1 months, P = 0.004, respectively). In the multivariate analysis, the high NANCI/high NKX2-1 duplex was identified as an independent prognostic factor for longer DFS (HR 0.346, 95% CI, 0.169–0.709; P = 0.004) and OS (HR 0.309, 95% CI, 0.121–0.786; P = 0.014).ConclusionsNANCI and the NANCI-NKX2-1 duplex impacts prognosis in stage I NSCLC patients.     

阿达木单抗治疗强直性脊柱炎致播散性结核病一例并文献复习

目的 探讨应用阿达木单克隆抗体(简称“单抗”)治疗强直性脊柱炎致播散性结核病的临床特点、诊治要点和治疗转归。 方法 回顾性分析福建省福州肺科医院2019年6月10日收治的1例应用阿达木单抗治疗强直性脊柱炎致播散性结核病的临床资料、诊治经过及随访情况,并进行文献复习。以“adalimumab”和“disseminated tuberculosis”为检索词对PubMed数据库进行检索,以“阿达木单抗”和“播散性结核病”为检索词通过万方数据库和中国知网对中文文献进行检索,检索时间截止到2019年9月,经过筛选剔除,共获取相关文献34篇,其中中文文献0篇,英文文献34篇。删去重复的文献并剔除可能为阿达木单抗以外的肿瘤坏死因子α(TNF-α)致播散性结核病及TNF-α致其他播散性疾病的报道,共筛选出具备详细病例资料的文献8篇,共获得8例患者,结合本研究收集的患者,对其临床特征、诊断和治疗情况进行分析。 结果 本例患者为男性,28岁。因“强直性脊柱炎”接受阿达木单抗治疗,治疗2个月后出现咳嗽、气促、腹胀、发热,入院后经胸部CT、胸膜活检及支气管镜等检查,诊断为“播散性结核病(双肺、右侧支气管、胸腔、腹腔、心包、纵隔淋巴结、锁骨上淋巴结、腹腔淋巴结、盆腔淋巴结、脾)”。给予“3H-R-Z-E/9H-R-E”方案治疗,辅以异烟肼支气管局部雾化吸入,行抗结核药物治疗后症状改善。治疗第5个月,CT复查提示“肺部病变减少,纵隔内部分淋巴结肿大较前缩小,增厚的支气管管壁较前变薄,管腔较前通畅,胸、腹腔积液明显吸收。截止到2019年12月,患者仍处于规则的抗结核药物治疗中。文献检索后获得8例患者,加上本例,共9例患者。其中,男3例,女6例;年龄9~75岁,平均年龄(50.44±25.19)岁。9例患者中,5例开始使用阿达木单抗治疗之前的结核病筛查试验结果为阴性,1例既往有抗结核治疗史,1例曾进行过预防性抗结核治疗,3例有与结核病患者的密切接触史。诊断明确行抗结核药物治疗后,5例患者转归良好。3例转归差,其中1例病情持续进展,并发消化道出血;1例颅内病灶持续进展;1例出现急性呼吸窘迫综合征,最终死亡。1例转归不明。 结论 阿达木单抗可致播散性结核病,准备接受阿达木单抗治疗的患者均应在用药前进行结核病筛查,治疗过程中应该警惕潜伏性结核感染转为活动性结核病及新发结核感染,停用阿达木单抗和及时行抗结核药物治疗是预后良好的关键。     

R-spondin signaling as a pivotal regulator of tissue development and homeostasis

R-spondins (Rspos) are cysteine-rich secreted glycoproteins which control a variety of cellular functions and are essential for embryonic development and tissue homeostasis. R-spondins (Rspo1 to 4) have high structural similarity and share 60% sequence homology. It has been shown that their cysteine-rich furin-like (FU) domain and the thrombospondin (TSP) type I repeat domain are essential for initiating downstream signaling cascades and therefore for their biological functions. Although numerous studies have unveiled their pivotal role as critical developmental regulators, the most important finding is that Rspos synergize Wnt signaling. Recent studies have identified novel receptors for Rspos, the Lgr receptors, closely related orphans of the leucin-rich repeat containing G protein-coupled receptors, and proposed that Rspos potentiate canonical Wnt signaling via these receptors. Given that Wnt signaling is one of the most important developmental signaling pathways that controls cell fate decisions and tissue development, growth and homeostasis, Rspos may function as key players for these processes as well as potential therapeutic targets. Here, I recapitulate the Wnt signaling and then outline the biological role of Rspos in tissue development and homeostasis and explore the possibility that Rspos may be used as therapeutic targets.     

酰胺质子转移成像在脑疾病中的研究进展

酰胺质子转移成像(ATP)是一种新的由化学饱和交换位移成像(CEST)发展而来的通过检测体内游离蛋白质或多肽中酰胺质子与水中的氢质子之间交换速率从而反映体内游离蛋白质含量及pH值变化的成像方式,能够在脑肿瘤、脑卒中、神经退行性疾病及儿童脑发育中提供重要的影像学信息,为临床的诊断、治疗及预后方面提供帮助。本文主要阐述APT成像方式在脑疾病当中的研究进展。     

Changes in physical activity,sedentary behaviour and sleep across the transition from primary to secondary school: A systematic review

ObjectivesTo describe how children’s time spent in the 24-h movement behaviours of physical activity (PA), sedentary behaviour (SB) and sleep change, individually and collectively, across the transition from primary to secondary school.DesignSystematic review.MethodsSix electronic databases were searched from January 1990 to May 2019. Eligibility criteria included longitudinal studies reporting time spent in PA, SB and/or sleep, with baseline assessments conducted during the last two years of primary school and at least one follow-up during the first two years of secondary school. For studies reporting only SB, this review considered those published from November 2015 onwards to update a previous systematic review.ResultsThe present review identified six articles that reported changes in PA (n = 5) or PA and SB concurrently (n = 1). Most articles had a high risk of bias (n = 4/6). There was limited but consistent evidence of a change in PA over the school transition period; in particular a decrease in total daily PA and during specific time periods (i.e., in-school, after-school and leisure time). A concurrent but opposite change was observed in SB. No studies were identified that assessed changes in sleep, or all three movement behaviours concurrently.ConclusionsFurther research exploring concurrent changes in all movement behaviours (PA, SB and sleep) and associated factors is warranted to inform future behavioural interventions and policies for promoting an optimal 24 h movement behaviour pattern during this critical developmental period.